DILUTING
Dilutions are generally performed in multiples of two or ten. This makes it easier to perform and to calculate concentrations. For example, a 1 to 10 dilution (written 1:10) is 1 volume of bacteria into a total of 10 volumes of liquid. This means that one volume of bacteria is added to 9 volumes of diluting liquid (making a total of 10 volumes).
How would you dilute a broth culture containing 100,000 organisms/ml to make one containing 100 organisms/ml ? You could take 1 ml of the culture and place it into 999 ml of water. For reasons of practicality and accuracy, dilutions of large numbers of bacteria utilize a serial dilution, series of smaller dilutions rather than one large dilution. The final dilution is calculated by multiplying each of the smaller dilutions. Thus, if 1 ml of culture is diluted into 9 ml of water (1:10) and 1 ml of this diluted material is added to another 9 ml of water (1:10), the final dilution will be 1:10 x 1:10 or 1:100. A third 1:10 dilution would give a final dilution of 1:1000.
(hentet fra et helt enkelt googlesøk)
Så kan de som jobber med saft få gjøre hva de vil, den blander jeg etter egen smak. Fortynning og 1:X er ikke oppe til diskusjon.